Recombinant Human HGF protein, C-His tag

Product Description

Cat

IMP-666

Official Symbol

HGF

Product Overview

Recombinant Human HGF(P14210)(Gln32-Ser728) protein, fused with a 6*His tag at the C-terminus, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Human

Tag

C-His tag

Form

Lyophilized from sterile 20mM Tris-HCl, 500mM NaCl, pH 8.0.

Molecular Mass

The protein has a calculated MW of 26&53.7kDa, and migrates as 32-38&55-65kDa under reducing conditions.

Protein length

Gln32-Ser728

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-Cell based assay 1

Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. The ED50 for this effect is 0.3-1.5 ng/ml.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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