Recombinant Human HGF protein, C-His tag

Product Description

Cat

IMP-666

Official Symbol

HGF

Product Overview

Recombinant Human HGF(P14210)(Gln32-Ser728) protein, fused with a 6*His tag at the C-terminus, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Human

Tag

C-His tag

Form

Lyophilized from sterile 20mM Tris-HCl, 500mM NaCl, pH 8.0.

Molecular Mass

The protein has a calculated MW of 26&53.7kDa, and migrates as 32-38&55-65kDa under reducing conditions.

Protein length

Gln32-Ser728

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-Cell based assay 1

Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. The ED50 for this effect is 0.3-1.5 ng/ml.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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