Product Overview
Biotinylated recombinant human HGF protein (Gln32-Ser728, Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile) without tag was expressed in Mouse myeloma cell line.
Description
This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss.
Protein length
Gln32-Ser728, Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile
Bio-activity
Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human HGF Prorein is captured on EvenCoat Streptavidin Coated Plates at 0.500 μg/mL (100 μL/well), Recombinant Human HGFR/c-MET Fc Chimera Protein binds with an ED50 of 6.00-60.0 ng/mL.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
