Product Overview
Recombinant human HGF protein (Gln32-Ser728, Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile) without tag was expressed in Mouse myeloma cell line and Manufactured and tested under cGMP guidelines.
Description
This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss.
Predicted N Terminal
Amino acid sequencing of the alpha chain was blocked, suggesting it is consistent with Gln32
Gln32-Arg-Lys-Arg-Arg-Asn-Thr-Ile-His-Glu (alpha-chain)
Val495-Val-Asn-Gly-Ile-Pro-Thr-Arg-Thr-Asn (beta-chain)
Molecular Mass
Predicted Molecular Mass: alpha chain: 53.7 kDa beta chain: 26 kDa
SDS-PAGE: 65-75 kDa, non-reducing conditions; 60-65 kDa, and 30-40 kDa, reducing conditions
Protein length
Gln32-Ser728, Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile
Bio-activity
Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is 0.1-0.5 ng/mL. The specific activity of recombinant human HGF is >1.0 x 106 IU/mg, which is calibrated against the human HGF WHO International Standard (NIBSC code: 96/564).
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
