Product Overview
Recombinant human HGF protein (Gln32-Ser728, Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile) without tag was expressed in Sf 21 cells.
Description
This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss.
Molecular Mass
Predicted Molecular Mass: 53.7 kDa (alpha chain), 26 kDa (beta chain)
SDS-PAGE: 60-70 kDa, non-reducing conditions; 60 kDa, and 33-34 kDa, reducing conditions
Protein length
Gln32-Ser728, Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile
Bio-activity
Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is 0.1-0.5 ng/mL. The specific activity of Recombinant Human HGF is approximately 1.5 x 103 IU/μg, which is calibrated against Recombinant Human HGF WHO International Standard (NIBSC code: 96/564).
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
