Recombinant Human HGF Protein (Gln32-Ser728)
Product Description
Cat
IMP-10398
Official Symbol
HGF
Product Overview
Recombinant human HGF Propeptide protein (Gln32-Ser728) without tag was expressed in Sf 21 cells.
Description
This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss.
Expression System
Sf 21 cells
Species
Human
Tag
Tag Free
Predicted N Terminal
No results obtained: Gln32 predicted, sequencing might be blocked
Form
Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl.
Molecular Mass
Predicted Molecular Mass: 79.7 kDa
SDS-PAGE: 84 kDa, reducing conditions
Protein length
Gln32-Ser728
Bio-activity
>20 ng of Recombinant Human HGF Propeptide is cleaved to release active HGF when treated for 4 hours at 37 centigrade with 5 ng of kallikrein.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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