Recombinant Human HLA-A Complex Tetramer protein, C-His-Avi Tag

Product Description

Cat

IMP-1630

Official Symbol

HLA-A

Product Overview

Recombinant Human HLA-A Complex Tetramer protein(A0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL)(Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and RMFPNAPYL peptide) is expressed from Expi293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.

Expression System

HEK293

Species

Human

Tag

C-His-Avi Tag

Form

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

Protein length

It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and RMFPNAPYL peptide.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Storage

-20 to -80°C for 12 months as supplied from date of receipt.
'-20 to -80°C for 3-6 months in unopened state after reconstitution.
4-8℃ for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.U17

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

SDS-PAGE

HPLC

Bioactivity-ELISA 1

Immobilized Human WT-1 (HLA-A*02:01) Complex Tetramer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Ab., hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.

Bioactivity-SPR 1

Anti-WT-1 (HLA-A*02:01) Antibody captured on Protein A chip, can bind Human WT-1 (HLA-A*02:01) Complex Tetramer, His Tag with an affinity constant of 0.18nM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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