Recombinant Human HLA-A Complex Tetramer protein, C-His-Avi Tag

Product Description

Cat

IMP-1630

Official Symbol

HLA-A

Product Overview

Recombinant Human HLA-A Complex Tetramer protein(A0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL)(Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and RMFPNAPYL peptide) is expressed from Expi293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.

Expression System

HEK293

Species

Human

Tag

C-His-Avi Tag

Form

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

Protein length

It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and RMFPNAPYL peptide.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Storage

-20 to -80°C for 12 months as supplied from date of receipt.
'-20 to -80°C for 3-6 months in unopened state after reconstitution.
4-8℃ for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.U17

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

SDS-PAGE

HPLC

Bioactivity-ELISA 1

Immobilized Human WT-1 (HLA-A*02:01) Complex Tetramer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Ab., hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.

Bioactivity-SPR 1

Anti-WT-1 (HLA-A*02:01) Antibody captured on Protein A chip, can bind Human WT-1 (HLA-A*02:01) Complex Tetramer, His Tag with an affinity constant of 0.18nM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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