Recombinant Human HLA-A Complex Tetramer protein, C-His-Avi Tag
Product Description
Cat
IMP-1646
Official Symbol
HLA-A
Product Overview
Recombinant Human HLA-A Complex Tetramer protein(A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse HLA-A*02:01 α3)&P61769(B2M)&RMFPNAPYL)(Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119 (B2M) and RMFPNAPYL peptide) is expressed from Expi293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin.
Expression System
HEK293
Species
Human
Tag
C-His-Avi Tag
Form
Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Molecular Mass
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Protein length
It contains Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119 (B2M) and RMFPNAPYL peptide.
Endotoxin
Less than 1EU per μg by the LAL method.
Purity
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Storage
Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE
Tris-Bis PAGE

HPLC
SEC-HPLC

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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