Recombinant Human HLA-A protein(NY-ESO-1), C-His-Avi Tag, Biotinylated

Product Description

Cat

IMP-1667

Official Symbol

HLA-A

Product Overview

Biotinylated Recombinant Human HLA-A protein(NY-ESO-1)(A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQV)(Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and SLLMWITQV peptide) is expressed from Expi293 with His tag and Avi tag at the C-terminal.

Expression System

HEK293

Species

Human

Tag

C-His-Avi Tag

Form

Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.

Molecular Mass

The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-56 kDa based on Tris-Bis PAGE result.

Protein length

It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and SLLMWITQV peptide.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Storage

Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

HPLC

Bioactivity-ELISA 1

Immobilized Biotinylated Human NY-ESO-1(HLA-A*02:01), His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-NY-ESO-1(HLA-A*02:01) Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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