Recombinant Human HLA-A protein(P53 WT), C-His-Avi Tag
Product Description
Cat
IMP-1671
Official Symbol
HLA-A
Product Overview
Recombinant Human HLA-A protein(P53 WT)(A0A140T913(HLA-A*02:01)&P61769(B2M)&HMTEVVRRC)(Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and HMTEVVRRC peptide) is expressed from Expi293 with His tag and Avi tag at the C-terminal.
Expression System
HEK293
Species
Human
Tag
C-His-Avi Tag
Form
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Molecular Mass
The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.
Protein length
It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and HMTEVVRRC peptide.
Endotoxin
Less than 1EU per μg by the LAL method.
Purity
> 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC
Storage
-20 to -80°C for 12 months as supplied from date of receipt. '-20 to -80°C for 3-6 months in unopened state after reconstitution. 4-8℃ for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.U17
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
