Recombinant Human HLA-A protein(P53 WT), C-His-Avi Tag
Product Description
Cat
IMP-1671
Official Symbol
HLA-A
Product Overview
Recombinant Human HLA-A protein(P53 WT)(A0A140T913(HLA-A*02:01)&P61769(B2M)&HMTEVVRRC)(Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and HMTEVVRRC peptide) is expressed from Expi293 with His tag and Avi tag at the C-terminal.
Expression System
HEK293
Species
Human
Tag
C-His-Avi Tag
Form
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Molecular Mass
The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.
Protein length
It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and HMTEVVRRC peptide.
Endotoxin
Less than 1EU per μg by the LAL method.
Purity
> 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC
Storage
-20 to -80°C for 12 months as supplied from date of receipt. '-20 to -80°C for 3-6 months in unopened state after reconstitution. 4-8℃ for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.U17
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
