Recombinant Human HLA-A Tetramer protein, C-His-Avi Tag

Product Description

Cat

IMP-1643

Official Symbol

HLA-A

Product Overview

Recombinant Human HLA-A Tetramer protein(A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse HLA-A*02:01 α3)&P61769(B2M)&CLGGLLTMV)(Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119(B2M) and CLGGLLTMV peptide) is expressed from Expi293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin.

Expression System

HEK293

Species

Human

Tag

C-His-Avi Tag

Form

Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.

Molecular Mass

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

Protein length

It contains Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119(B2M) and CLGGLLTMV peptide.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Storage

Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

HPLC

Bioactivity-ELISA 1

Immobilized Human&Mouse Chimeric LMP2 (HLA-A*02:01) Tetramer, His Tag at 2μg/ml(100μl/Well) on the plate. Dose response curve for Anti-LMP2(HLA-A*02:01) Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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