Recombinant Human HLA-DRB1 Protein (30-227aa), N-His-Tagged
Product Description
Cat
IMP-9350
Official Symbol
HLA-DRB1
Product Overview
Recombinant human HLA-DRB1 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Description
HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail.
Expression System
E. coli
Species
Human
Tag
N-His
Form
Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Molecular Mass
25.2 kDa (219 aa)
Protein length
30-227
AA Sequence
MGSSHHHHHHSSGLVPRGSHMGDTRPRFLWQPKRECHFFNGTERVRFLDRYFYNQEESVRFDSDVGEFRAVTELGRPDAEYWNSQKDILEQARAAVDTYCRHNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVSGFYPGSIEVRWFLNGQEEKAGMVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRARSESAQSK
Purity
> 85% by SDS-PAGE
Applications
SDS-PAGE,Denatured
Notes
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/mL (determined by Bradford assay)
SDS-PAGE
SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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