Recombinant Human HLA-DRB1 Protein (30-227aa), N-His-Tagged
Product Description
Cat
IMP-9350
Official Symbol
HLA-DRB1
Product Overview
Recombinant human HLA-DRB1 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Description
HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail.
Expression System
E. coli
Species
Human
Tag
N-His
Form
Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Molecular Mass
25.2 kDa (219 aa)
Protein length
30-227
AA Sequence
MGSSHHHHHHSSGLVPRGSHMGDTRPRFLWQPKRECHFFNGTERVRFLDRYFYNQEESVRFDSDVGEFRAVTELGRPDAEYWNSQKDILEQARAAVDTYCRHNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVSGFYPGSIEVRWFLNGQEEKAGMVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRARSESAQSK
Purity
> 85% by SDS-PAGE
Applications
SDS-PAGE,Denatured
Notes
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/mL (determined by Bradford assay)
SDS-PAGE
SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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