Recombinant Human HLA-G Complex Tetramer protein, C-His-Avi Tag
Product Description
Cat
IMP-1618
Official Symbol
HLA-G
Product Overview
Recombinant Human HLA-G Complex Tetramer protein(P17693-1(HLA-G)&P61769(B2M)&RIIPRHLQL)(Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide) is expressed from Expi293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.
Expression System
HEK293
Species
Human
Tag
C-His-Avi Tag
Form
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Molecular Mass
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Protein length
It contains Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide.
Endotoxin
Less than 1EU per μg by the LAL method.
Purity
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
'-20 to -80°C for 3-6 months in unopened state after reconstitution.
4-8℃ for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.U17
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
SDS-PAGE
Tris-Bis PAGE

HPLC
SEC-HPLC

Bioactivity-ELISA 1
Blocking Data

Serial dilutions of Anti-LILRB2 Antibody were added into HLA-G Complex Tetramer, His Tag : Human LILRB2, mFc Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 70.0ng/ml.

Bioactivity-SPR 1
SPR Data

Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-G Complex Tetramer with an affinity constant of 4.62 nM as determined in SPR assay (Biacore T200).

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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