Recombinant Human HLA-G Complex Tetramer protein, C-His-Avi Tag, PE-Labeled

Product Description

Cat

IMP-1624

Official Symbol

HLA-G

Product Overview

PE-Labeled Recombinant Human HLA-G Complex Tetramer Protein(P17693-1(HLA-G)&P61769(B2M)&RIIPRHLQL)(Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide) is expressed from Expi293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.

Expression System

HEK293

Species

Human

Tag

C-His-Avi Tag

Form

Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.

Molecular Mass

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

Protein length

It contains Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE

Storage

Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Serial dilutions of Anti-LILRB2 Antibody were added into PE-Labeled Human HLA-G Complex Tetramer, His Tag : Human LILRB2, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.45μg/ml.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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