IMP-10400

HSPG2

Recombinant human Endorepellin protein (Arg3684-Ser4391, Ser4004Thr) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

This gene encodes the perlecan protein, which consists of a core protein to which three long chains of glycosaminoglycans (heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin, prolargin, collagen type IV, FGFBP1, FBLN2, FGF7 and transthyretin, etc., and it plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix, where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes, where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1, Silverman-Handmaker type of dyssegmental dysplasia, and tardive dyskinesia. Alternative splicing of this gene results in multiple transcript variants.

Mouse myeloma cell line

Human

C-10×His

Arg3684

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Predicted Molecular Mass: 76.7 kDa
SDS-PAGE: 90-95 kDa, reducing conditions

Arg3684-Ser4391, Ser4004Thr

Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF R1/Flt-1 is coated at 5 μg/mL (100 μL/well), the concentration of Recombinant Human Endorepellin/Perlecan that produces 50% of the optimal binding response is approximately 0.75-6 μg/mL.

<0.10 EU/μg of the protein by the LAL method.

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitute at 200 μg/mL in PBS.

Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF R1/Flt-1 is coated at 5 μg/mL (100 μL/well), the concentration of Recombinant Human Endorepellin/Perlecan that produces 50% of the optimal binding response is approximately 0.75-6 μg/mL.