Recombinant Human ICAM1 Protein (Gln28-Glu480), C-hFc and Avi-tagged
Product Description
Cat
IMP-10409
Official Symbol
ICAM1
Product Overview
Recombinant human ICAM-1/CD54 protein (Gln28-Glu480) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Human embryonic kidney cell.
Description
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
Expression System
HEK293
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Gln28 inferred from enzymatic pyroglutamate treatment revealing Thr29
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 78 kDa
SDS-PAGE: 95-125 kDa, under reducing conditions
Protein length
Gln28-Glu480
Bio-activity
Measured by its binding ability in a functional ELISA. When Human ICAM-1 (14C11) Antibody is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human ICAM-1/CD54 Fc Chimera Avi-tag binds with an ED50 of 3-18 ng/mL. Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. The ED50 for this effect is 0.3-2.7 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Human ICAM-1 (14C11) Antibody is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human ICAM-1/CD54 Fc Chimera Avi-tag binds with an ED50 of 3-18 ng/mL. Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. The ED50 for this effect is 0.3-2.7 μg/mL.

Data Sheet MSDS
Datasheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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