Recombinant Human ICAM1 Protein (Gln28-Glu480), C-hFc and Avi-tagged

Product Description

Cat

IMP-10409

Official Symbol

ICAM1

Product Overview

Recombinant human ICAM-1/CD54 protein (Gln28-Glu480) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Human embryonic kidney cell.

Description

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.

Expression System

HEK293

Species

Human

Tag

C-hFc and Avi

Predicted N Terminal

Gln28 inferred from enzymatic pyroglutamate treatment revealing Thr29

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Molecular Mass

Predicted Molecular Mass: 78 kDa
SDS-PAGE: 95-125 kDa, under reducing conditions

Protein length

Gln28-Glu480

Bio-activity

Measured by its binding ability in a functional ELISA. When Human ICAM-1 (14C11) Antibody is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human ICAM-1/CD54 Fc Chimera Avi-tag binds with an ED50 of 3-18 ng/mL. Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. The ED50 for this effect is 0.3-2.7 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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