Recombinant Human ICOS Protein (Glu21-Gln134), C-6×His-tagged

Product Description

Cat

IMP-10411

Official Symbol

ICOS

Product Overview

Recombinant human ICOS protein (Glu21-Gln134) with a 6×His tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

Expression System

CHO

Species

Human

Tag

C-6×His

Predicted N Terminal

Glu21

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Molecular Mass

Predicted Molecular Mass: 14 kDa
SDS-PAGE: 20-34 kDa, reducing conditions

Protein length

Glu21-Gln134

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human B7-H2 Fc Chimera at 0.5 μg/mL (100 μL/well) can bind Recombinant Human ICOS with an ED50 of 25-125 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Noncovalent Homodimer

Storage

12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human B7-H2 Fc Chimera at 0.5 μg/mL (100 μL/well) can bind Recombinant Human ICOS with an ED50 of 25-125 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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