Recombinant Human ICOS Protein (Glu21-Gln134), C-6×His-tagged
Product Description
Cat
IMP-10411
Official Symbol
ICOS
Product Overview
Recombinant human ICOS protein (Glu21-Gln134) with a 6×His tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.
Expression System
CHO
Species
Human
Tag
C-6×His
Predicted N Terminal
Glu21
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 14 kDa
SDS-PAGE: 20-34 kDa, reducing conditions
Protein length
Glu21-Gln134
Bio-activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human B7-H2 Fc Chimera at 0.5 μg/mL (100 μL/well) can bind Recombinant Human ICOS with an ED50 of 25-125 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Noncovalent Homodimer
Storage
12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human B7-H2 Fc Chimera at 0.5 μg/mL (100 μL/well) can bind Recombinant Human ICOS with an ED50 of 25-125 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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