Recombinant Human ICOS Protein (Glu21-Gln134), C-hFc and Avi-tagged, Biotinylated

Product Description

Cat

IMP-10413

Official Symbol

ICOS

Product Overview

Biotinylated recombinant human ICOS protein (Glu21-Gln134) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

Expression System

CHO

Species

Human

Tag

C-hFc and Avi

Predicted N Terminal

Glu21

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 41 kDa
SDS-PAGE: 49-62 kDa, under reducing conditions

Protein length

Glu21-Gln134

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H2 Fc Chimera Protein is coated at 100 ng/mL (100 μL/well), the concentration of Biotinylated Recombinant Human ICOS Fc Chimera Avi-tag that produces 50% of the optimal binding response is approximately 0.80-4.80 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer, biotinylated via Avi-tag

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 250 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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