Recombinant Human ICOS Protein (Glu21-Lys140), C-6×His-tagged

Product Description

Cat

IMP-10412

Official Symbol

ICOS

Product Overview

Recombinant human ICOS protein (Glu21-Lys140) with a 6×His tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

Expression System

CHO

Species

Human

Tag

C-6×His

Predicted N Terminal

Glu21

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Molecular Mass

Predicted Molecular Mass: 15 kDa
SDS-PAGE: 18-36 kDa, reducing conditions

Protein length

Glu21-Lys140

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H2 Fc Chimera is coated at 0.5 μg/mL, 100 μL/well, Recombinant Human ICOS binds with an ED50 of 0.7-3.5 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H2 Fc Chimera is coated at 0.5 μg/mL, 100 μL/well, Recombinant Human ICOS binds with an ED50 of 0.7-3.5 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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