Recombinant Human ICOS Protein (Glu21-Phe141), C-hFc-tagged, Biotinylated
Product Description
Cat
IMP-10406
Official Symbol
ICOS
Product Overview
Biotinylated recombinant human ICOS protein (Glu21-Phe141) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-hFc
Predicted N Terminal
Glu21
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 40 kDa (unlabeled)
Protein length
Glu21-Phe141
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H2 Fc Chimera Recombinant Human B7-H2 Fc Chimera is immobilized at 0.1 μg/mL (100 μL/well), Biotinylated Recombinant Human ICOS Fc Chimera binds with an ED50 of 1-6 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 400 μg/mL in PBS.
Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H2 Fc Chimera Recombinant Human B7-H2 Fc Chimera is immobilized at 0.1 μg/mL (100 μL/well), Biotinylated Recombinant Human ICOS Fc Chimera binds with an ED50 of 1-6 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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