Recombinant Human ICOS Protein (Glu21-Phe141), C-hFc-tagged, Biotinylated
Product Description
Cat
IMP-10406
Official Symbol
ICOS
Product Overview
Biotinylated recombinant human ICOS protein (Glu21-Phe141) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-hFc
Predicted N Terminal
Glu21
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 40 kDa (unlabeled)
Protein length
Glu21-Phe141
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H2 Fc Chimera Recombinant Human B7-H2 Fc Chimera is immobilized at 0.1 μg/mL (100 μL/well), Biotinylated Recombinant Human ICOS Fc Chimera binds with an ED50 of 1-6 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 400 μg/mL in PBS.
Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H2 Fc Chimera Recombinant Human B7-H2 Fc Chimera is immobilized at 0.1 μg/mL (100 μL/well), Biotinylated Recombinant Human ICOS Fc Chimera binds with an ED50 of 1-6 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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