Recombinant Human ICOS Protein (Glu21-Phe141), C-hFc-tagged

Product Description

Cat

IMP-10405

Official Symbol

ICOS

Product Overview

Recombinant human ICOS protein (Glu21-Phe141) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-hFc

Predicted N Terminal

Glu21

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 40.3 kDa (monomer)
SDS-PAGE: 50-60 kDa, reducing conditions

Protein length

Glu21-Phe141

Bio-activity

Measured by its ability to inhibit human T cell proliferation induced by rhB7-H2/Fc Chimera in the presence of anti-CD3. Wang, S. et al. (2000) Blood 96:2808. The ED50 for this effect is 1-4 μg/mL in the presence of 3 μg/mL Recombinant Human B7-H2 Fc Chimera and at least 20 ng/mL of anti-CD3. Optimal dilutions should be determined by each laboratory for each application.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

Online Inquiry