Recombinant Human ICOSLG Protein (Ala19-Thr139), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10607
Official Symbol
ICOSLG
Product Overview
Biotinylated recombinant human B7-H2 Isoform 3 protein (Ala19-Thr139) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Human embryonic kidney cell.
Description
Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.
Expression System
HEK293
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Ala19
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 42 kDa
SDS-PAGE: 61-70 kDa, under reducing conditions
Protein length
Ala19-Thr139
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human ICOS Fc Chimera is immobilized at 10 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant B7-H2 Isoform 3 Fc Chimera Avi-tag that produces 50% of the optimal binding response is approximately 10-50 μg/mL.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 400 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human ICOS Fc Chimera is immobilized at 10 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant B7-H2 Isoform 3 Fc Chimera Avi-tag that produces 50% of the optimal binding response is approximately 10-50 μg/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
Phone
  • 1-631-559-9269
    1-516-512-3133
Fax
  • 1-631-938-8127
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