Recombinant Human ICOSLG Protein (Ala19-Thr139), C-hFc and Avi-tagged, Biotinylated

Product Description

Cat

IMP-10607

Official Symbol

ICOSLG

Product Overview

Biotinylated recombinant human B7-H2 Isoform 3 protein (Ala19-Thr139) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Human embryonic kidney cell.

Description

Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.

Expression System

HEK293

Species

Human

Tag

C-hFc and Avi

Predicted N Terminal

Ala19

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 42 kDa
SDS-PAGE: 61-70 kDa, under reducing conditions

Protein length

Ala19-Thr139

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human ICOS Fc Chimera is immobilized at 10 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant B7-H2 Isoform 3 Fc Chimera Avi-tag that produces 50% of the optimal binding response is approximately 10-50 μg/mL.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer, biotinylated via Avi-tag

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 400 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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