Recombinant Human ICOSLG Protein (Ala19-Thr139), C-hFc-tagged

Product Description

Cat

IMP-10421

Official Symbol

ICOSLG

Product Overview

Recombinant human B7-H2 Isoform 3 protein (Ala19-Thr139) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-hFc

Predicted N Terminal

Ala19

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Molecular Mass

Predicted Molecular Mass: 40 kDa
SDS-PAGE: 57-66 kDa, reducing conditions

Protein length

Ala19-Thr139

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human ICOS Fc Chimera is immobilized at 5 μg/mL, Recombinant Human B7-H2 Isoform 3 Fc Chimera binds with an ED50 of 10-50 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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