Recombinant Human ICOSLG Protein (Asp19-Ser258), C-Fc-tagged, Alexa Fluor 488 Conjugated

Product Description

Cat

IMP-10124

Official Symbol

ICOSLG

Product Overview

Alexa Fluor 488 conjugated recombinant human B7-H2 protein (Asp19-Ser258) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Labeled with Alexa Fluor 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm

Description

Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-Fc

Predicted N Terminal

Asp19

Form

Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Conjugate

Alexa Fluor 488

Molecular Mass

Predicted Molecular Mass: 53.4 kDa (monomer)
SDS-PAGE: 80-90 kDa, under reducing conditions.

Protein length

Asp19-Ser258

Bio-activity

Measured by flow cytometry for its ability to bind anti-human B7-H2 Antibody conjugated beads. The concentration of Recombinant Human B7-H2 Fc Chimera Alexa Fluor 488 that produces 50% of the binding response is 20.0-200 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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