Recombinant Human ICOSLG Protein (Asp19-Ser258), C-Fc-tagged, Alexa Fluor 488 Conjugated

Product Description

Cat

IMP-10124

Official Symbol

ICOSLG

Product Overview

Alexa Fluor 488 conjugated recombinant human B7-H2 protein (Asp19-Ser258) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Labeled with Alexa Fluor 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm

Description

Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-Fc

Predicted N Terminal

Asp19

Form

Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Conjugate

Alexa Fluor 488

Molecular Mass

Predicted Molecular Mass: 53.4 kDa (monomer)
SDS-PAGE: 80-90 kDa, under reducing conditions.

Protein length

Asp19-Ser258

Bio-activity

Measured by flow cytometry for its ability to bind anti-human B7-H2 Antibody conjugated beads. The concentration of Recombinant Human B7-H2 Fc Chimera Alexa Fluor 488 that produces 50% of the binding response is 20.0-200 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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