Recombinant Human ICOSLG Protein (Asp19-Ser258), C-hFc and Avi-tagged, Biotinylated

Product Description

Cat

IMP-10422

Official Symbol

ICOSLG

Product Overview

Biotinylated recombinant human B7-H2 protein (Asp19-Ser258) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.

Expression System

CHO

Species

Human

Tag

C-hFc and Avi

Predicted N Terminal

Asp19

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 55 kDa
SDS-PAGE: 80-100 kDa, under reducing conditions

Protein length

Asp19-Ser258

Bio-activity

The biotin to protein ratio is 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human ICOS Fc Chimera Protein is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human B7-H2 Fc Chimera Avi-tag that produces a 50% optimal binding response is found to be 1-6 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer, biotinylated via Avi-tag

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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