Recombinant Human ICOSLG Protein (Asp19-Ser258), C-hFc and Avi-tagged, Biotinylated

Product Description

Cat

IMP-10422

Official Symbol

ICOSLG

Product Overview

Biotinylated recombinant human B7-H2 protein (Asp19-Ser258) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.

Expression System

CHO

Species

Human

Tag

C-hFc and Avi

Predicted N Terminal

Asp19

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 55 kDa
SDS-PAGE: 80-100 kDa, under reducing conditions

Protein length

Asp19-Ser258

Bio-activity

The biotin to protein ratio is 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human ICOS Fc Chimera Protein is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human B7-H2 Fc Chimera Avi-tag that produces a 50% optimal binding response is found to be 1-6 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer, biotinylated via Avi-tag

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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