Recombinant Human IDO1 Protein (Ala2-Gly403), N-6×His-tagged
Product Description
Cat
IMP-10664
Official Symbol
IDO1
Product Overview
Recombinant human IDO protein (Ala2-Gly403) with a 6×His tag at N-terminus was expressed in E. coli.
Description
This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.
Expression System
E. coli
Species
Human
Tag
N-6×His
Predicted N Terminal
Met
Form
Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol.
Molecular Mass
Predicted Molecular Mass: 46 kDa
SDS-PAGE: 42 kDa, reducing conditions
Protein length
Ala2-Gly403
Bio-activity
Measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine. The specific activity is >500 pmol/min/μg, as measured under the described conditions.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Monomer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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