Recombinant Human IDO1 Protein (Ala2-Gly403), N-6×His-tagged
Product Description
Cat
IMP-10664
Official Symbol
IDO1
Product Overview
Recombinant human IDO protein (Ala2-Gly403) with a 6×His tag at N-terminus was expressed in E. coli.
Description
This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.
Expression System
E. coli
Species
Human
Tag
N-6×His
Predicted N Terminal
Met
Form
Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol.
Molecular Mass
Predicted Molecular Mass: 46 kDa
SDS-PAGE: 42 kDa, reducing conditions
Protein length
Ala2-Gly403
Bio-activity
Measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine. The specific activity is >500 pmol/min/μg, as measured under the described conditions.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Monomer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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