Recombinant Human IDO1 Protein Full Length, His-tagged

Product Description

Cat

IMP-6460

Official Symbol

IDO1

Product Overview

Recombinant IDO1 was expressed in E. coli cells as the full length protein (accession number NP_002155.1) with an N-terminal 6xHis Tag. The molecular weight of the protein is 47.5 kDa.

Description

IDO1 (Indoleamine 2,3-dioxygenase 1, also known as IDO, INDO, or IDO-1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan. IDO1 acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin.

Expression System

E. coli

Species

Human

Tag

His

Form

Recombinant IDO1 protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol and 0.5 mM TCEP.

Molecular Mass

47.5 kDa

Purity

>90%

Applications

Enzyme kinetics, screening inhibitors, and selectivity profiling

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

SDS-PAGE

Recombinant IDO1 protein gel 10% SDS-PAGE Coomassie staining MW: 47.5 kDa Purity: > 90%

Bioactivity-ELISA 1

Recombinant IDO1 protein activity assay 400 μM L-tryptophan was incubated with different concentrations of IDO1 protein in reaction buffer mixture. The reaction was stopped by the addition of 40 μL 30% (w/v) trichloroacetic acid and heated at 65 centigrade for 15 min. After cooling down to 4 centigrade, the reaction was centrifuged at 1,125 g for 10 min. 100 μL supernatant was transferred into 96-well plate and mixed with 100 μL of 2% (w/v) p-DMAB. The yellow pigment derived from kynurenine was measured at 490 nm.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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