Recombinant Human IDO1 Protein Full Length, His-tagged

Product Description

Cat

IMP-6460

Official Symbol

IDO1

Product Overview

Recombinant IDO1 was expressed in E. coli cells as the full length protein (accession number NP_002155.1) with an N-terminal 6xHis Tag. The molecular weight of the protein is 47.5 kDa.

Description

IDO1 (Indoleamine 2,3-dioxygenase 1, also known as IDO, INDO, or IDO-1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan. IDO1 acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin.

Expression System

E. coli

Species

Human

Tag

His

Form

Recombinant IDO1 protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol and 0.5 mM TCEP.

Molecular Mass

47.5 kDa

Purity

>90%

Applications

Enzyme kinetics, screening inhibitors, and selectivity profiling

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

SDS-PAGE

Recombinant IDO1 protein gel 10% SDS-PAGE Coomassie staining MW: 47.5 kDa Purity: > 90%

Bioactivity-ELISA 1

Recombinant IDO1 protein activity assay 400 μM L-tryptophan was incubated with different concentrations of IDO1 protein in reaction buffer mixture. The reaction was stopped by the addition of 40 μL 30% (w/v) trichloroacetic acid and heated at 65 centigrade for 15 min. After cooling down to 4 centigrade, the reaction was centrifuged at 1,125 g for 10 min. 100 μL supernatant was transferred into 96-well plate and mixed with 100 μL of 2% (w/v) p-DMAB. The yellow pigment derived from kynurenine was measured at 490 nm.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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