Description
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Molecular Mass
Predicted Molecular Mass: 104 kDa (single chain), 81 kDa (alpha subunit) and 23 kDa (beta subunit)
SDS-PAGE: 150 kDa, 120 kDa and 48 kDa, reducing conditions
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human IGF-I R/IGF1R is present at 0.5 μg/mL, the concentration of Recombinant Human IGF-I/IGF-1 that produces 50% of the optimal binding response is found to be approximately 2-10 ng/mL.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
