Recombinant Human IgG1Fc(Asp239Glu,Leu241Glu) protein, No tag

Product Description

Cat

IMP-1111

Official Symbol

IgG1Fc

Product Overview

Recombinant Human IgG1Fc(P01857)(Asp104-Lys330 (Asp239Glu,Leu241Glu)) protein, fused with no tag, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Human

Tag

No tag

Form

Lyophilized from sterile PBS, pH 7.4.

Molecular Mass

The protein has a calculated MW of 25.9kDa, and migrates as 32kDa under reducing conditions.

Protein length

Asp104-Lys330 (Asp239Glu, Leu241Glu)

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human IgG1 Fc at 2μg/ml (100 μl/well) can bind Human CD64-His. The ED50 of Human IgG1 Fc is 30-150 ng/ml .

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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