Recombinant Human IL12RB1 Protein (Cys24-Glu540), C-hFc and 6×His-tagged
Product Description
Cat
IMP-10500
Official Symbol
IL12RB1
Product Overview
Recombinant human IL-12 R beta 1 protein (Cys24-Glu540) with Human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-hFc and 6×His
Predicted N Terminal
Cys24
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 84.5 kDa (monomer)
SDS-PAGE: 110 kDa, reducing conditions
Protein length
Cys24-Glu540
Bio-activity
Measured by its binding ability in a functional ELISA. Immobilized rhIL-12 R beta 1/Fc Chimera at 5 μg/mL (100 μL/well) can bind rhIL-12 with a linear range of 0.78-50 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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