Recombinant Human IL12RB1 Protein (Cys24-Glu540), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10502
Official Symbol
IL12RB1
Product Overview
Biotinylated recombinant human IL-12 R beta 1 protein (Cys24-Glu540) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants.
Expression System
CHO
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Cys24
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 85 kDa
SDS-PAGE: 95-115 kDa, under reducing conditions
Protein length
Cys24-Glu540
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human IL-12 is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL-12 beta 1 Fc Chimera Avi-tag that produces 50% of the optimal binding response is found to be approximately 15-90 ng/mL.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-12 is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL-12 beta 1 Fc Chimera Avi-tag that produces 50% of the optimal binding response is found to be approximately 15-90 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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