Recombinant Human IL12RB1 Protein (Cys24-Glu540), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10502
Official Symbol
IL12RB1
Product Overview
Biotinylated recombinant human IL-12 R beta 1 protein (Cys24-Glu540) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants.
Expression System
CHO
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Cys24
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 85 kDa
SDS-PAGE: 95-115 kDa, under reducing conditions
Protein length
Cys24-Glu540
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human IL-12 is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL-12 beta 1 Fc Chimera Avi-tag that produces 50% of the optimal binding response is found to be approximately 15-90 ng/mL.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-12 is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL-12 beta 1 Fc Chimera Avi-tag that produces 50% of the optimal binding response is found to be approximately 15-90 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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