Recombinant Human IL13RA1 Protein (Ala27-Thr343, Thr130Ile), C-hFc and 6×His-tagged

Product Description

Cat

IMP-10499

Official Symbol

IL13RA1

Product Overview

Recombinant human IL-13 R alpha 1 protein (Ala27-Thr343, Thr130Ile) with Human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-hFc and 6×His

Predicted N Terminal

Ala27

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 64 kDa (monomer)
SDS-PAGE: 110 kDa, reducing conditions

Protein length

Ala27-Thr343, Thr130Ile

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized rhIL-13 R alpha 1/Fc Chimera at 4 μg/mL (100 μL/well) can bind rhIL-13 with a linear range of 1-100 ng/mL. Optimal dilutions should be determined by each laboratory for each application.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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