Recombinant Human IL15 Protein, Animal-Free, GMP-Grade
Product Description
Cat
IMP-10614
Official Symbol
IL15
Product Overview
Recombinant human IL-15 protein without tag was expressed in E. coli, produced using non-animal reagents in an animal-free laboratory and manufactured and tested under cGMP guidelines.
Description
The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported.
Expression System
E. coli
Species
Human
Tag
Tag Free
Predicted N Terminal
Asn-Trp-Val-Asn-Val-Ile-Ser-Asp-Leu-Lys
Form
Lyophilized from an phosphate buffered saline-based Formulation using proprietary excipients.
Molecular Mass
Predicted Molecular Mass: 12.5 kDa
Bio-activity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.3-2.6 ng/mL. The specific activity of ProDots Recombinant Human IL-15 GMP is >2.0 x 108 units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554).
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>97%, by SDS-PAGE with silver staining.
Storage
Store material as supplied, unopened, and unreconstituted at 2-8 centigrade until use. Stability is a minimum of 8 weeks when stored at 2-8 centigrade as supplied. Refer to lot specific COA for the Use by Date.
The foil pouch is used for shipping and storage only and is not terminally sterilized. GMP ProDots within the weldable bag are prepared under GMP-controlled conditions and protein content is tested to USP <71> guidelines.
Reconstitution
Reconstitute immediately prior to use with up to 25 mL of cell culture media.
Bioactivity-ELISA 1
Binding Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.3-2.6 ng/mL. The specific activity of ProDots Recombinant Human IL-15 GMP is >2.0 x 108 units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554).

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
Phone
  • 1-631-559-9269
    1-516-512-3133
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