Recombinant Human IL15 Protein, No Tag
Product Description
Cat
IMP-4642
Official Symbol
IL15
Product Overview
Recombinant human IL15 (NP_000576.1) (Asn49-Ser162) was expressed.
Description
The protein encoded by the IL15 gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietic receptor subunits and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively, spliced transcript variants of this gene have been reported.
Expression System
E. coli
Species
Human
Tag
No Tag
Predicted N Terminal
Asn 49
Form
Lyophilized from sterile 50 mM Tris, 200 mM NaCl, pH 8.0, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human IL15 consists of 114 amino acids and predicts a molecular mass of 12.8 kDa.
Protein length
Asn49-Ser162
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-BLI 1

Labeled biotin to IL-15 Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of IL-15 Protein, Human, Recombinant, Biotinylated bound to Anti-IL15 Antibody was 0.1nM.

Bioactivity-Cell based assay 1
Bioactivity-Cell based assay

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The EC50 for this effect is typically 2-8 ng/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
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