Recombinant Human IL17A protein, C-His tag

Product Description

Cat

IMP-1128

Official Symbol

IL17A

Product Overview

Recombinant Human IL17A(Q16552)(Gly24-Ala155) protein, fused with a 6*His tag at the C-terminus, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Human

Tag

C-His tag

Form

Lyophilized from sterile PBS, pH 7.4.

Molecular Mass

The protein has a calculated MW of 15.9kDa, and migrates as 15-19kDa under reducing conditions.

Protein length

Gly24-Ala155

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-Cell based assay 1

Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 6-60 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry