Recombinant Human IL17A Protein, Tag free

Product Description

Cat

IMP-8499

Official Symbol

IL17A

Product Overview

Human IL17A (Q16552) recombinant protein expressed in E. coli.

Description

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Expression System

E. coli

Species

Human

Tag

Tag free

Form

Lyophilized, No additive

Molecular Mass

31 kDa

AA Sequence

MIVKAGITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVA

Endotoxin

< 0.1 EU/μg

Applications

Functional Study; SDS-PAGE

Storage

Store at -20 centigrade on dry atmosphere. After reconstitution with sterilized water, store at -20 centigrade. Aliquot to avoid repeated freezing and thawing.

SDS-PAGE

1 μg/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue Lane 1: non-reducing conditions Lane 2: reducing conditions

Bioactivity-ELISA 1

Serial dilutions of human IL17A (starting at 800 ng/mL) were added to NIH 3T3 cells. After 48 hours, production of mouse IL-6 was measured and the linear portion of the curve was us used to calculate the ED50.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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