Recombinant Human IL17B Protein (Gln21-Phe180)
Product Description
Cat
IMP-10497
Official Symbol
IL17B
Product Overview
Recombinant human IL-17B protein (Gln21-Phe180) without tag was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a T cell-derived cytokine that shares sequence similarity with IL17. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies. Alternative splicing results in multiple splice variants.
Expression System
CHO
Species
Human
Tag
Tag Free
Predicted N Terminal
Gln21, Ser51&Gly102
Gln21 predicted, sequencing might be blocked
Form
Lyophilized from a 0.2 μm filtered solution in Sodium Phosphate and NaCl with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 18 kDa (monomer)
SDS-PAGE: 20-25 kDa, reducing conditions
Protein length
Gln21-Phe180
Bio-activity
Measured by its ability to induce IL-8 secretion by HepG2 human hepatocellular carcinoma cells. The ED50 for this effect is 0.4-2 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Noncovalently-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to induce IL-8 secretion by HepG2 human hepatocellular carcinoma cells. The ED50 for this effect is 0.4-2 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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