Recombinant Human IL17F Protein (Arg31-Gln163), C-6×His-tagged

Product Description

Cat

IMP-10529

Official Symbol

IL17F

Product Overview

Recombinant human IL-17F protein (Arg31-Gln163) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-6×His

Predicted N Terminal

Arg31

Form

Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 15.7 kDa (monomer)
SDS-PAGE: 21 kDa, reducing conditions

Protein length

Arg31-Gln163

Bio-activity

Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 2-10 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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