Recombinant Human IL17RA(Q96F46)(Leu33-Trp320) protein, fused with a Fc tag at the C-terminus, was expressed from HEK293 Cells.
Expression System
HEK293
Species
Human
Tag
C-hFc tag
Form
Lyophilized from sterile PBS, pH 7.4.
Molecular Mass
The protein has a calculated MW of 60.6kDa, and migrates as 90-120 kDa under reducing conditions.
Protein length
Leu33-Trp320
Endotoxin
Less than 1.0 EU per ug as determined by the LAL method.
Purity
>95% as determined by reducing SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Bioactivity-Cell based assay 1
Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 20-100 ng/ml.
