Recombinant Human IL17RA Protein (Leu33-Trp320), C-6×His-tagged

Product Description

Cat

IMP-10505

Official Symbol

IL17RA

Product Overview

Recombinant human IL-17RA/IL-17R protein (Leu33-Trp320) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.

Description

Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The transmembrane protein encoded by this gene (interleukin 17A receptor; IL17RA) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Alternative splicing results in multiple transcript variants encoding different isoforms.

Expression System

CHO

Species

Human

Tag

C-6×His

Predicted N Terminal

Leu33

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Molecular Mass

Predicted Molecular Mass: 34 kDa
SDS-PAGE: 60-68 kDa, under reducing conditions.

Protein length

Leu33-Trp320

Bio-activity

Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.050-1.00 μg/mL in the presence of 10.0 ng/mL recombinant human IL-17.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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