Recombinant Human IL17RA Protein (Leu33-Trp320), C-6×His-tagged
Product Description
Cat
IMP-10505
Official Symbol
IL17RA
Product Overview
Recombinant human IL-17RA/IL-17R protein (Leu33-Trp320) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The transmembrane protein encoded by this gene (interleukin 17A receptor; IL17RA) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Alternative splicing results in multiple transcript variants encoding different isoforms.
Expression System
CHO
Species
Human
Tag
C-6×His
Predicted N Terminal
Leu33
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 34 kDa
SDS-PAGE: 60-68 kDa, under reducing conditions.
Protein length
Leu33-Trp320
Bio-activity
Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.050-1.00 μg/mL in the presence of 10.0 ng/mL recombinant human IL-17.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Reconstitution
Reconstitute at 250 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.050-1.00 μg/mL in the presence of 10.0 ng/mL recombinant human IL-17.

Data Sheet MSDS
Datasheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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