Recombinant Human IL1a protein, N-His Tag

Product Description

Cat

IMP-2535

Official Symbol

IL1a

Product Overview

Recombinant Human IL1a protein(Q53QF9)(Ser113~Ala271), fused with N-terminal His Tag, was expressed in E. coli.

Expression System

E. coli

Species

Human

Tag

N-His Tag

Form

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Molecular Mass

21kDa

Protein length

Leu80~Leu235

Purity

> 97%

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Western Blot 1

SDS-PAGE

Bioactivity-ELISA 1

Inhibitory effect of IL1α on cell proliferation of MCF-7 cells

(A)MCF-7 cells cultured in DMEM, stimulted with 1ng/mL IL1α for 72h. (B)Unstimulated MCF-7 cells cultured in DMEM for 72h.

Bioactivity-ELISA 2

Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with various concentrations of IL1α for 72h. The mean OD value of MCF-7 assessed by CCK-8 was shown in Figure 2. It was obvious that IL1α significantly decreased cell viability of MCF-7 cells.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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