Recombinant Human IL1a protein, N-His Tag

Product Description

Cat

IMP-2535

Official Symbol

IL1a

Product Overview

Recombinant Human IL1a protein(Q53QF9)(Ser113~Ala271), fused with N-terminal His Tag, was expressed in E. coli.

Expression System

E. coli

Species

Human

Tag

N-His Tag

Form

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Molecular Mass

21kDa

Protein length

Leu80~Leu235

Purity

> 97%

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Western Blot 1

SDS-PAGE

Bioactivity-ELISA 1

Inhibitory effect of IL1α on cell proliferation of MCF-7 cells

(A)MCF-7 cells cultured in DMEM, stimulted with 1ng/mL IL1α for 72h. (B)Unstimulated MCF-7 cells cultured in DMEM for 72h.

Bioactivity-ELISA 2

Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with various concentrations of IL1α for 72h. The mean OD value of MCF-7 assessed by CCK-8 was shown in Figure 2. It was obvious that IL1α significantly decreased cell viability of MCF-7 cells.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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