Recombinant Human IL1A(P01583)(Ser113-Ala271) protein, fused with no tag, was expressed from E.coli.
Expression System
E.coli
Species
Human
Tag
No tag
Form
Lyophilized from sterile 20mM TrisHCl, 150mM NaCl, pH 7.5.
Molecular Mass
The protein has a calculated MW of 18kDa, and migrates as 17kDa under reducing conditions.
Protein length
Ser113-Ala271
Endotoxin
Less than 1.0 EU per ug as determined by the LAL method.
Purity
>95% as determined by reducing SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
Bioactivity-Cell based assay 1
Measured by its ability to induce NFKB reporter gene expression in HEK 293 cell line.
