Recombinant Human IL1RL2 Protein (Asp20-Tyr337), C-hFc-tagged
Product Description
Cat
IMP-10495
Official Symbol
IL1RL2
Product Overview
Recombinant human IL-1Rrp2/IL-1R6 protein (Asp20-Tyr337) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Sf 21 cells.
Description
The protein encoded by this gene is a member of the interleukin 1 receptor family. An experiment with transient gene expression demonstrated that this receptor was incapable of binding to interleukin 1 alpha and interleukin 1 beta with high affinity. This gene and four other interleukin 1 receptor family genes, including interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 1 (IL1RL1), and interleukin 18 receptor 1 (IL18R1), form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
Expression System
Sf 21 cells
Species
Human
Tag
C-hFc
Predicted N Terminal
Asp20
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 62.8 kDa (monomer)
SDS-PAGE: 80 kDa, reducing conditions
Protein length
Asp20-Tyr337
Bio-activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human (rh) IL-1F5, rhIL-1F8, rhIL-1F6 or rhIL-1F9 at 1 μg/mL (100 μL/well) can bind rhIL-1Rrp2 Fc Chimera with a linear range of 0.15-5 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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