Recombinant Human IL2 Protein, C-His-Avi tag, Biotinylated

Product Description

Cat

IMP-976

Official Symbol

IL2

Product Overview

Biotinylated Recombinant Human IL2(P60568)(Ala21-Thr153) Protein, fused with a 6*His, Avi tag at the C-terminus, is expressed from HEK293 cells.

Expression System

HEK293

Species

Human

Tag

C-His-Avi tag

Form

Lyophilized from sterile PBS, pH 7.4.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 18.3kDa, and migrates as 16-18kDa under reducing conditions.

Protein length

Ala21-Thr153

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Notes

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-Cell based assay 1

Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 0.2 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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