Recombinant Human IL2 Protein, C-His-Avi tag, Biotinylated

Product Description

Cat

IMP-976

Official Symbol

IL2

Product Overview

Biotinylated Recombinant Human IL2(P60568)(Ala21-Thr153) Protein, fused with a 6*His, Avi tag at the C-terminus, is expressed from HEK293 cells.

Expression System

HEK293

Species

Human

Tag

C-His-Avi tag

Form

Lyophilized from sterile PBS, pH 7.4.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 18.3kDa, and migrates as 16-18kDa under reducing conditions.

Protein length

Ala21-Thr153

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Notes

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-Cell based assay 1

Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 0.2 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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