Recombinant Human IL2 Protein, No Tag

Product Description

Cat

IMP-4692

Official Symbol

IL2

Product Overview

Recombinant mature form of human IL2 (P60568-1) (Ala 21-Thr 153) was expressed and purified with an initial Met.

Description

Interleukin-2, also known as a T-cell growth factor, TCGF, Aldesleukin, and IL2, is a secreted protein that belongs to the IL-2 family. Interleukin-2 / IL-2 was the first interleukin molecule to be discovered. Interleukin-2 / IL-2 molecule was first purified to homogeneity by immunoaffinity chromatography by Kendall Smith and his team at Dartmouth Medical School. Interleukin-2 / IL-2 was also the first cytokine shown to mediate its effects via a specific IL-2 receptor, and it was also the first interleukin to be cloned and expressed from a complementary DNA (cDNA) library. Interleukin-2 / IL-2 was designated number 2 because Smith's data at the time indicated that IL-1, produced by macrophages, facilitates IL-2 production by T lymphocytes (T cells).

Expression System

E. coli

Species

Human

Tag

No Tag

Predicted N Terminal

Met

Form

Supplied as sterile 20mM HAc-NaAc+0.004% Tween-80 pH 4.0

Molecular Mass

The recombinant human IL-2 consists of 134 amino acids and predicts a molecular mass of 15.5 kDa.

Protein length

Ala21-Thr153

Endotoxin

< 5 EU/mg of the protein.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Bioactivity-BLI 1

Using the Octet RED System, the affinity constant (Kd) of IL2 Protein, Human, Recombinant bound Anti-IL2 Antibody was 0.1 nM.

Bioactivity-Cell based assay 1

Measured in a cell proliferation assay using CTLL2. The ED50 for this effect is typically 1-8 ng/mL. The specific activity of recombinant human IL-2 is approximately 20, 000 IU/μg.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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