Recombinant Human IL25 Protein (Tyr33-Gly177)
Product Description
Cat
IMP-10556
Official Symbol
IL25
Product Overview
Recombinant human IL-17E protein (Tyr33-Gly177) without tag was expressed in Human embryonic kidney cell.
Description
The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants.
Expression System
HEK293
Species
Human
Tag
Tag Free
Predicted N Terminal
Tyr33
Form
Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 17 kDa (monomer)
SDS-PAGE: 20-28 kDa, reducing conditions
Protein length
Tyr33-Gly177
Bio-activity
Measured by its ability to induce CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cells. The ED50 for this effect is 0.25-1.5 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE with silver staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its ability to induce CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cells. The ED50 for this effect is 0.25-1.5 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info