Recombinant Human IL25 Protein (Tyr33-Gly177)
Product Description
Cat
IMP-10556
Official Symbol
IL25
Product Overview
Recombinant human IL-17E protein (Tyr33-Gly177) without tag was expressed in Human embryonic kidney cell.
Description
The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants.
Expression System
HEK293
Species
Human
Tag
Tag Free
Predicted N Terminal
Tyr33
Form
Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 17 kDa (monomer)
SDS-PAGE: 20-28 kDa, reducing conditions
Protein length
Tyr33-Gly177
Bio-activity
Measured by its ability to induce CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cells. The ED50 for this effect is 0.25-1.5 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE with silver staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its ability to induce CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cells. The ED50 for this effect is 0.25-1.5 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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