Recombinant Human IL2RA Protein (Glu22-Cys213), C-Avi and 6×His-tagged
Product Description
Cat
IMP-10536
Official Symbol
IL2RA
Product Overview
Recombinant human CD25/IL-2R alpha protein (Glu22-Cys213) with a Avi-tag and 6×His tag at C-terminus was expressed in Human embryonic kidney cell.
Description
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk.
Expression System
HEK293
Species
Human
Tag
C-Avi and 6×His
Predicted N Terminal
Glu22
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 25 kDa
SDS-PAGE: 36-51 kDa, under reducing conditions
Protein length
Glu22-Cys213
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human IL-2 is immobilized at 1 μg/mL (100 μL/well), Biotinylated Recombinant Human CD25/IL-2R alpha His-tag Avi-tag binds with an ED50 of 45.0-540 ng/mL. The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-2 is immobilized at 1 μg/mL (100 μL/well), Biotinylated Recombinant Human CD25/IL-2R alpha His-tag Avi-tag binds with an ED50 of 45.0-540 ng/mL. The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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