Recombinant Human IL2RG Protein (Leu23-Asn254), C-6×His-tagged

Product Description

Cat

IMP-10545

Official Symbol

IL2RG

Product Overview

Recombinant human Common gamma Chain protein (Leu23-Asn254) with a C-terminal 6×His tag was expressed in Sf 21 cells.

Description

The protein encoded by this gene is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.

Expression System

Sf 21 cells

Species

Human

Tag

C-6×His

Predicted N Terminal

Leu23

Form

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 28.2 kDa
SDS-PAGE: 40 kDa, reducing conditions

Protein length

Leu23-Asn254

Bio-activity

Measured by its ability to inhibit the IL-2-dependent proliferation of MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.6-3.6 μg/mL in the presence of 30 μg/mL of soluble IL-2 R beta and 10 ng/mL of recombinant human IL-2.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

Online Inquiry