Recombinant Human IL2RG Protein (Leu23-Asn254), C-6×His-tagged

Product Description

Cat

IMP-10545

Official Symbol

IL2RG

Product Overview

Recombinant human Common gamma Chain protein (Leu23-Asn254) with a C-terminal 6×His tag was expressed in Sf 21 cells.

Description

The protein encoded by this gene is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.

Expression System

Sf 21 cells

Species

Human

Tag

C-6×His

Predicted N Terminal

Leu23

Form

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 28.2 kDa
SDS-PAGE: 40 kDa, reducing conditions

Protein length

Leu23-Asn254

Bio-activity

Measured by its ability to inhibit the IL-2-dependent proliferation of MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.6-3.6 μg/mL in the presence of 30 μg/mL of soluble IL-2 R beta and 10 ng/mL of recombinant human IL-2.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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