Recombinant Human IL2RG Protein (Leu23-Asn254), C-hFc and Avi-tagged, Biotinylated

Product Description

Cat

IMP-10547

Official Symbol

IL2RG

Product Overview

Biotinylated recombinant human Common gamma Chain protein (Leu23-Asn254) with a Human IgG1 Fc (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

The protein encoded by this gene is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.

Expression System

CHO

Species

Human

Tag

C-hFc and Avi

Predicted N Terminal

Leu23

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 56 kDa
SDS-PAGE: 85-95 kDa, under reducing conditions.

Protein length

Leu23-Asn254

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD25/IL-2R alpha Protein and Recombinant Human IL-2R beta Protein are immobilized at 5.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Common gamma Chain/IL-2 R gamma Fc Chimera Avi-tag that produces 50% of the optimal binding response is 0.250-2.50 μg/mL in the presence of Recombinant Human IL-2 Protein.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer, biotinylated via Avi-tag

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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