Recombinant Human IL2RG Protein (Leu23-Asn254), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10547
Official Symbol
IL2RG
Product Overview
Biotinylated recombinant human Common gamma Chain protein (Leu23-Asn254) with a Human IgG1 Fc (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.
Expression System
CHO
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Leu23
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 56 kDa
SDS-PAGE: 85-95 kDa, under reducing conditions.
Protein length
Leu23-Asn254
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD25/IL-2R alpha Protein and Recombinant Human IL-2R beta Protein are immobilized at 5.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Common gamma Chain/IL-2 R gamma Fc Chimera Avi-tag that produces 50% of the optimal binding response is 0.250-2.50 μg/mL in the presence of Recombinant Human IL-2 Protein.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD25/IL-2R alpha Protein and Recombinant Human IL-2R beta Protein are immobilized at 5.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Common gamma Chain/IL-2 R gamma Fc Chimera Avi-tag that produces 50% of the optimal binding response is 0.250-2.50 μg/mL in the presence of Recombinant Human IL-2 Protein.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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