Recombinant Human IL36RN(Q9UBH0)(Val2-Asp155) protein, fused with no tag, was expressed from E.coli.
Expression System
E.coli
Species
Human
Tag
No tag
Form
Lyophilized from sterile PBS, pH 7.4.
Molecular Mass
The protein has a calculated MW of 16.8kDa, and migrates as 17kDa under reducing conditions.
Protein length
Val2-Asp155
Endotoxin
Less than 1.0 EU per ug as determined by the LAL method.
Purity
>95% as determined by reducing SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Bioactivity-Cell based assay 1
Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 47.1 ng/ml.
