Recombinant Human IL3RA Protein (Lys20-Arg305)
Product Description
Cat
IMP-10450
Official Symbol
IL3RA
Product Overview
Recombinant human IL-3R alpha/CD123 protein (Lys20-Arg305) without tag was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found.
Expression System
Mouse myeloma cell line
Species
Human
Tag
Tag Free
Predicted N Terminal
Lys20
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 33 kDa
Protein length
Lys20-Arg305
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human IL-3 R alpha/CD123 is present at 1 μg/mL, the concentration of Recombinant Human IL-3 that produces 50% of the optimal binding response is found to be approximately 8-40 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-3 R alpha/CD123 is present at 1 μg/mL, the concentration of Recombinant Human IL-3 that produces 50% of the optimal binding response is found to be approximately 8-40 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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